elisa試劑盒的檢測前準備工作及注意事項

<p class="MsoNormal"><strong><span style="color: rgb(255, 0, 0);"><span style="font-family: 宋體; font-size: 9pt;">檢測前準備工作：</span></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1.</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"> <font face="宋體">請提前</font><font face="Calibri">20</font><font face="宋體">分鐘從冰箱中取出試劑盒，以平衡至室溫。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2. </span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">將濃縮洗滌液用雙蒸水稀釋（</font><font face="Calibri">1:20</font><font face="宋體">）。未用完的放回</font><font face="Calibri">4</font><font face="宋體">℃。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">3.</span></strong><span style="font-size: 9pt;"> <font face="宋體" style="font-family: 宋體;">標準品</font></span><span style="font-family: 宋體;">：</span><span style="font-size: 9pt;"><font face="宋體" style="font-family: 宋體;">加入標準品</font><font face="Calibri" style="font-family: 宋體;">/</font><font face="宋體" style="font-family: 宋體;">標本稀釋液</font><font face="Calibri" style="font-family: 宋體;">0.5ml</font><font face="宋體" style="font-family: 宋體;">至凍干標準品中，靜置</font><font face="Calibri" style="font-family: 宋體;">15</font><font face="宋體" style="font-family: 宋體;">分鐘，待其充分溶解后，輕輕混勻（濃度為</font><font face="Calibri" style="font-family: 宋體;">1000 pg/ml</font><font face="宋體" style="font-family: 宋體;">）。然后根據需要進行稀釋。（建議標準曲線使用以下濃度： </font><font face="Calibri" style="font-family: 宋體;">1000</font><font face="宋體" style="font-family: 宋體;">、</font><font face="Calibri" style="font-family: 宋體;">500</font><font face="宋體" style="font-family: 宋體;">、</font><font face="Calibri" style="font-family: 宋體;">250</font><font face="宋體" style="font-family: 宋體;">、</font><font face="Calibri" style="font-family: 宋體;">125</font><font face="宋體" style="font-family: 宋體;">、</font><font face="Calibri" style="font-family: 宋體;">62.5</font><font face="宋體" style="font-family: 宋體;">、</font><font face="Calibri" style="font-family: 宋體;">31.25</font><font face="宋體" style="font-family: 宋體;">、</font><font face="Calibri" style="font-family: 宋體;">15.6</font><font face="宋體" style="font-family: 宋體;">、</font><font face="Calibri" style="font-family: 宋體;">0 pg/ml</font><font face="宋體" style="font-family: 宋體;">）。復溶標準品原液（</font><font face="Calibri" style="font-family: 宋體;">1000 pg/ml</font><font face="宋體" style="font-family: 宋體;">）若未用完請分裝后放入</font><font face="Calibri" style="font-family: 宋體;">-20</font><font face="宋體" style="font-family: 宋體;">℃以下電冰箱內保存，保存兩個月。已稀釋的標準品請廢棄。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">4.</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"> <font face="宋體">生物素化抗體工作液：按當次試驗所需要得用量，使用前</font><font face="Calibri">20</font><font face="宋體">分鐘，以生物素化抗體稀釋液稀釋濃縮生物素化抗體（</font><font face="Calibri">1:30</font><font face="宋體">）成工作濃度。當日使用。若實驗次數過多導致生物素化 抗體量不足，可向我們公司單獨購買生物素化抗體。（須提供抗體批號）</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">5.</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"> <font face="宋體">酶結合物工作液：按當次試驗所需要得用量，使用前</font><font face="Calibri">20</font><font face="宋體">分鐘，以酶結合物稀釋液稀釋濃 縮酶結合物（</font><font face="Calibri">1:30</font><font face="宋體">） 成工作濃度。當日使用。若實驗次數過多導致濃縮酶結合物量不足， 可向我們公司單獨購買濃縮酶結合物。（須提供酶結合物批號）</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">洗滌方法：</span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1. <font face="宋體">自動洗板機：要求注入的洗滌液為</font><font face="Calibri">350ul</font><font face="宋體">，注入與吸出間隔</font><font face="Calibri">15</font><font face="宋體">－</font><font face="Calibri">30</font><font face="宋體">秒。洗板</font><font face="Calibri">5</font><font face="宋體">次。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2. <font face="宋體">手工洗板：甩盡孔內液體，在潔凈的吸水紙上拍干，每孔加洗滌液</font><font face="Calibri">350ul</font><font face="宋體">，靜置</font><font face="Calibri">30</font><font face="宋體">秒后甩盡液體，在厚迭吸水紙上拍干。洗板</font><font face="Calibri">5</font><font face="宋體">次。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">注意事項：</span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1. <font face="宋體">試劑盒使用前請保存在</font><font face="Calibri">2-8</font><font face="宋體">℃。復溶后的標準品應分裝后，將其放在</font><font face="Calibri">-20</font><font face="宋體">～</font><font face="Calibri">-70</font><font face="宋體">℃貯存。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2. <font face="宋體">濃縮生物素化抗體，濃縮酶結合物體積小，運輸中顛簸和可能的倒置，會使液體沾到管壁 或瓶蓋。因此使用前請用手甩幾下或</font><font face="Calibri">1000</font><font face="宋體">轉</font><font face="Calibri">/</font><font face="宋體">分離心</font><font face="Calibri">1</font><font face="宋體">分鐘，以使附著管壁或瓶蓋的液體沉積到管底。取用前，請用移液器小心吹打</font><font face="Calibri">4-5</font><font face="宋體">次使溶液混勻。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">3. <font face="宋體">從冰箱中取出的濃縮洗滌液可能有結晶</font><font face="Calibri">,</font><font face="宋體">屬于正常現象，微加熱至</font><font face="Calibri">40</font><font face="宋體">℃使結晶完全溶解后 再配制洗滌液。（加熱溫度不要超過</font><font face="Calibri">50</font><font face="宋體">℃）使用時洗滌液應為室溫。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">4. <font face="宋體">若需要分次使用標準品應按照每一次用量分裝，將其放在</font><font face="Calibri">-20</font><font face="宋體">～</font><font face="Calibri">-70</font><font face="宋體">℃貯存，避免反復凍 融。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">5. <font face="宋體">不同批號的試劑盒組份不能混用（洗滌液和反應終止液除外）。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">6. <font face="宋體">充分輕微混勻對反應結果尤為重要，使用微量振蕩器（使用</font><font face="Calibri">*</font><font face="宋體">頻率），如無微量振蕩器，可在反應前手工輕輕敲擊酶標板框混勻。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">7. <font face="宋體">在試驗中標準品和樣本檢測時建議作雙孔檢測。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">8. <font face="宋體">試驗中所用的試管和吸頭均為一次性使用，嚴禁在不同的液體之間混用！否則將影響試 驗結果。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">9. <font face="宋體">試驗中請穿著試驗服并帶乳膠手套做好防護工作，特別是檢測血液或者其他體液標本時， 請按國家生物試驗室安全防護條列執行。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>