白介素類的elisa試劑盒實驗條件

<p class="MsoNormal"><span style="background-color: rgb(0, 255, 255);"><strong><span style="font-family: 宋體; font-size: 9pt;">實驗條件</span></strong></span><span style="font-family: 宋體; font-size: 9pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="font-family: 宋體; font-size: 9pt;">elisa<font face="宋體">試劑盒</font></span></strong><span style="font-family: 宋體; font-size: 9pt;"><font face="宋體">白介素類檢測抗體多用間接型</font><font face="Calibri">elisa</font><font face="宋體">，以純化抗原包被載體，經</font><font face="Calibri">4</font><font face="宋體">℃過夜，以無關蛋白封閉至少</font><font face="Calibri">2</font><font face="宋體">小時以上，加入待測樣品，室溫孵育</font><font face="Calibri">1</font><font face="宋體">小時，洗滌干凈后加酶標抗體，再經室溫孵育、洗滌后，以底物顯色，顯色到一定程度終止反應，在酶標儀上讀結果或以肉眼判斷。</font></span><span style="font-family: 宋體; font-size: 9pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">固相載體：</span></strong></span><span style="font-family: 宋體; font-size: 9pt;"><o:p></o:p></span></p> <p><font face="宋體" style="font-family: 宋體; font-size: 9pt;">可作為固相載體的物質很多，如：纖維素、聚丙烯酰胺、聚苯乙烯、聚丙烯、交聯葡聚糖、玻璃、硅橡膠、瓊脂糖凝膠等。可以各種形式出現；試管、微量反應板凹孔、小珠</font><span style="font-family: 宋體; font-size: 9pt;">.....</span><font face="宋體" style="font-family: 宋體; font-size: 9pt;">常用的是聚苯乙烯制品，其吸附性</font><font face="宋體" style="font-family: 宋體; font-size: 9pt;">好，固相包被的機制一般不外乎物理吸附和共價交聯，</font><font face="宋體" style="font-family: 宋體; font-size: 9pt;">類</font><span style="font-family: 宋體; font-size: 9pt;"> &nbsp;</span><font face="宋體" style="font-family: 宋體; font-size: 9pt;">型：</font></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">elisa<font face="宋體">具體方法的類型繁多，試劑的標準化問題還未</font><font face="Calibri">zui</font><font face="宋體">后解決，溫育的時間、溫度、洗滌液的種類及洗滌次數等都是影響測定的因素。但參考以上基本過程，</font></span><strong><span style="font-family: 宋體; font-size: 9pt;">elisa<font face="宋體">試劑盒</font></span></strong><font face="宋體" style="font-family: 宋體; font-size: 9pt;">無論怎樣變化，均離不開抗原</font><font face="Calibri" style="font-size: 9pt;">-</font><font face="宋體" style="font-family: 宋體; font-size: 9pt;">抗體反應、酶標抗體的免疫學反應及酶催化作用。</font></p> <p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;"><font face="宋體">特</font> &nbsp;<font face="宋體">點：</font></span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1<font face="宋體">、敏感性高，可檢出濃度在</font><font face="Calibri">ug~mg/ml</font><font face="宋體">水平；</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2<font face="宋體">、特異性強；</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">3<font face="宋體">、重復性好；</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">4<font face="宋體">、快速、簡便、廉價。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">封閉：</span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">以無關蛋白占據載體上未被包被的空隙，常用的封閉液是</font>1%<font face="宋體">牛血清白蛋白或</font><font face="Calibri">10%</font><font face="宋體">小牛血清。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">待測樣品：</span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">稀釋度要合適，濃度太大會發生非特異性吸附造成假陽性，過分稀釋會影響抗原</font>-<font face="宋體">抗體之間的免疫學反應。選擇</font><font face="Calibri">zui</font><font face="宋體">適稀釋度，在實驗體系中取光密度在</font><font face="Calibri">1.0</font><font face="宋體">左右的稀釋度為宜。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">酶結合物：</span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="font-family: 宋體; font-size: 9pt;">elisa<font face="宋體">試劑盒</font></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">使用高質量的酶結合物是</font>elisa<font face="宋體">成功的關鍵。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>